Browsing by Author "Aworunse, Oluwadurotimi S."

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Cross-Species Transferability of Cowpea SSR Markers to Vigna radiata (L.) (Mung Bean) and Phaseolus lunatus (L.) (Lima Bean)
(IOP Conf. Series: Earth and Environmental Science 1492, 2025) Obembe, Olawole O.; Ogundipe, Iyanuoluwa; Aworunse, Oluwadurotimi S.; Oyatomi, Olaniyi A.
Simple Sequence Repeats (SSRs) are tandemly repeated sequences of 1-6 nucleotide motifs found in all genomes. They constitute a considerable portion of the noncoding region of eukaryotic genome. The transferability of SSR markers across related species can be leveraged to assess genetic diversity, particularly in crops lacking species-specific DNA markers. Considering the close evolutionary relationships within the legume family, 10 cowpea (Vigna unguiculata) SSR markers were screened for their transferability across genomic DNA from 10 accessions each of Mung bean (Vigna radiata) and Lima bean (Phaseolus lunatus) using polymerase chain reaction (PCR). The results indicated that 70% of the markers were successfully amplified in Mung bean and 90% in Lima bean, while one specific marker (SSR- 7061) failed to amplify in any of the accessions. DNA markers, SSR-7079, VM-40, VM-54, and VM-74 had an amplification success rate ranging from 70% to 100% across the accessions. On the other hand, SSR-7000 and SSR-7053-2 had 55% and 50% amplification rates, respectively, while SSR-6313, SSR-37, and VM-71 exhibited less than 50% amplification across the accessions. The study generated 21 alleles from these markers, with allele counts per marker ranging from 1 to 3 and an average of 2.10 alleles per locus. The polymorphic information content values varied between 0.00 to 0.57 with an average value of 0.32, indicating moderate variability, while gene diversity measurements ranged from 0.16 to 0.64 with an average diversity value of 0.39. Regarding genetic relationships among the accessions, cluster analysis using the unweighted pair group method (UPGMA) revealed two main groups. Factorial analysis divided the accessions into four groups, with TPI-2428, TPI-2432, TVr-46 and TVr-6 forming distinct outliers. This cross-species applicability not only reinforces the conservation of genetic markers among legumes but also underscores the potential for these markers to assist in the breeding and genetic improvement of Mung bean and Lima bean.
Dataset on rbcL-based intra-specific diversity and population structure of Parkia biglobosa (Jacq.) in Nigeria
(Data in Brief (Elsevier), 2024) Omonhinmin, Conrad A.; Taiwo, Nchedo S.; Okonkwor. Paul B.; Ajayi, Israel M.; Akinduti, Paul Akinniyi; Aworunse, Oluwadurotimi S.; Ajiboye, Ibukun; Taiwo, Olugbenga S.; Adekeye, Bosede Temitope; Oziegbe, Olubukola; Bello, Adetutu O.; Ahuekwe, Eze Frank; Oyekanmi, Joshua; Olufowobi, Olanrewaju; Oniha, Margaret Ikhiwili; Oshamika, Oyewumi; Ejoh, Samuel A.; Adewale, Adeyemi G.; Akinnola, Olayemi O.; Oranusi, Solomon U.; Popoola, Jacob O.
African locust bean ( Parkia biglobosa ) is a multipurpose legu- minous tree species of nutritional and pharmacological value. The plant is widely distributed in Africa and across Nige- ria’s major agroecological areas (AEAs). Amidst declining cul- tivation and production, P. biglobosa is genetically threatened in its natural habitats due to overexploitation, deforestation, wildfires and lack of improved tree management practices. Consequently, concerted research efforts directed towards germplasm collection and assessment of genetic relation- ships are imperative for conserving its genetic resources, sustainable management and selecting promising landraces for breeding programmes. The dataset presents rbcL in- traspecific genetic diversity and population structure of 62 P. biglobosa landraces in Nigeria. A relatively high level of diversity and a low degree of nucleotide variability was observed among the landraces. Relatively high values of 642 total allele sites, 601 polymorphic sites, 504 parsimony information sites, 883 total number mutations, 9 haplotypes and 0.55 gene diversity were recorded for the sequence dataset. Low values of 0.35 nucleotide diversity and 5 InDels events were also recorded for the dataset. The gene flow in this dataset demonstrated an extensive exchange of genes between the three populations of P. biglobosa, which influenced the level of genetic differentiation (Gst) between the populations. Significantly low Gst (-0.01) was recorded between the Guinea and Sudan savannah populations, a moderate value (0.03) was recorded between the Sudan savannah and Rainforest populations and a higher Gst value (0.05) was recorded between the Guinea and Rainforest populations. The dataset highlights potential evolutionary dynamics that might influence variations relevant to the breeding and conservation of P. biglobosa in Nigeria and across its range in West and Central Africa.
Marine Greens as Nutritious Healthy Foods
(2025) Akinhanmi, Fadekemi O.; Aworunse, Oluwadurotimi S.; Ayanda, Opeyemi I.; Agiriga, Emmanuel