Browsing by Author "Obembe, Olawole O."
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Item Advancing Food Sustainability in Nigeria: Biotechnological Strategies amidst Climate Change Challenges(Tropical Journal of Natural Product Research, 2025, Vol 9, Issue 4, p1654, 2025-04) Obembe, Olawole O.; Obadire, Tobi T.;; Tawari, Princess P.-M. P.The effects of climate change are not just a looming threat but a present reality already being felt worldwide. One of the most visible and alarming impacts is the disruption of our food systems, caused by rising temperatures, changing precipitation patterns, and more frequent extreme weather events. These disruptions are leading to lower crop yields, food shortages, and higher prices, raising the spectre of a global food crisis that threatens the security of all nations. This study explores biotechnology's role in mitigating climate change and advancing sustainable agriculture in Nigeria. This work highlights how these approaches can significantly increase agricultural production, enhance food security, and reduce greenhouse gas emissions by leveraging innovative biotechnological techniques such as genetic engineering, biogas, biochar, bioremediation, and precision agriculture. For instance, genetic engineering improves crop resilience to environmental stresses, biochar enhances soil fertility while sequestering carbon, and biogas provides sustainable energy solutions. The findings emphasize the transformative potential of biotechnology despite challenges such as safety concerns and limited skilled personnel in Nigeria. By adopting these innovative techniques, Nigeria can enhance agricultural productivity, ensure food security, and actively contribute to global climate change mitigation efforts.Item Bioinformatics, Healthcare Informatics and Analytics: An Imperative for Improved Healthcare System(Foundation of Computer Science FCS, New York, USA) Obembe, Olawole O.Item Cellulose-hemicellulose networks as target for in planta modification of the properties of natural fibres(Academic Journals) Obembe, Olawole O.Item Comparative Analyses for Functional Food in Cucurbita pepo L. (Pumpkin) and Telfairia occidentalis HOOK F. (Fluted Pumpkin).(International Journal of Horticultural Science & Technology, 2026, Vol 13, Issue 3, p425, 2025) Obembe, Olawole O.; OBADIRE, Tobi Tejumade;; Ayankoya, Peace IfeoluwaTelfairia occidentalis and Cucurbita pepo, both members of the Cucurbitaceae family, are recognized for their considerable dietary importance. In this study, the two species were comparatively analyzed for their nutritional composition, phytochemical content, and mineral profiles to evaluate their potential as functional foods. Aqueous leaf extracts were subjected to qualitative phytochemical screening, with quantification of total alkaloids, saponins, tannins, flavonoids, and phenols. Standard analytical methods were employed for both mineral and proximate composition analyses. Phytochemical screening indicated the presence of alkaloids, flavonoids, and tannins in the leaf extracts of both T. occidentalis and C. pepo, whereas phenols were detected only in T. occidentalis. All statistical analyses were conducted at a confidence level of a = 0.05. In terms of proximate composition, T. occidentalis exhibited significantly higher protein, fiber, and ash contents, while C. pepo had significantly greater moisture and fat contents. Both species presented high carbohydrate levels, with no significant differences observed. Mineral analysis revealed that C. pepo contained significantly higher concentrations (mg kg-1) of calcium, potassium, copper, iron, and nitrate, emphasizing its value as a cost-effective nutritional resource. These findings suggest that T. occidentalis possesses greater pharmaceutical potential due to its richer phytochemical profile, whereas C. pepo exhibits superior nutritional qualities. This distinction highlights their complementary roles in the development of functional foods. Further studies are recommended to investigate the physiological effects of C. pepo and the potential synergistic benefits of combining both species in functional food applications.Item Cross-Species Transferability of Cowpea SSR Markers to Vigna radiata (L.) (Mung Bean) and Phaseolus lunatus (L.) (Lima Bean)(IOP Conf. Series: Earth and Environmental Science 1492, 2025) Obembe, Olawole O.; Ogundipe, Iyanuoluwa; Aworunse, Oluwadurotimi S.; Oyatomi, Olaniyi A.Simple Sequence Repeats (SSRs) are tandemly repeated sequences of 1-6 nucleotide motifs found in all genomes. They constitute a considerable portion of the noncoding region of eukaryotic genome. The transferability of SSR markers across related species can be leveraged to assess genetic diversity, particularly in crops lacking species-specific DNA markers. Considering the close evolutionary relationships within the legume family, 10 cowpea (Vigna unguiculata) SSR markers were screened for their transferability across genomic DNA from 10 accessions each of Mung bean (Vigna radiata) and Lima bean (Phaseolus lunatus) using polymerase chain reaction (PCR). The results indicated that 70% of the markers were successfully amplified in Mung bean and 90% in Lima bean, while one specific marker (SSR- 7061) failed to amplify in any of the accessions. DNA markers, SSR-7079, VM-40, VM-54, and VM-74 had an amplification success rate ranging from 70% to 100% across the accessions. On the other hand, SSR-7000 and SSR-7053-2 had 55% and 50% amplification rates, respectively, while SSR-6313, SSR-37, and VM-71 exhibited less than 50% amplification across the accessions. The study generated 21 alleles from these markers, with allele counts per marker ranging from 1 to 3 and an average of 2.10 alleles per locus. The polymorphic information content values varied between 0.00 to 0.57 with an average value of 0.32, indicating moderate variability, while gene diversity measurements ranged from 0.16 to 0.64 with an average diversity value of 0.39. Regarding genetic relationships among the accessions, cluster analysis using the unweighted pair group method (UPGMA) revealed two main groups. Factorial analysis divided the accessions into four groups, with TPI-2428, TPI-2432, TVr-46 and TVr-6 forming distinct outliers. This cross-species applicability not only reinforces the conservation of genetic markers among legumes but also underscores the potential for these markers to assist in the breeding and genetic improvement of Mung bean and Lima bean.Item Differential expression of cellulose synthase (CesA) gene transcripts in potato as revealed by QRT-PCR(Elsevier) Obembe, Olawole O.Item Endogenous levels of reducing sugars, free amino acids and phenols during various stages of in vitro culture of cotton (Gossypium Spp.)(International Formulae Group) Obembe, Olawole O.Item Evaluating Pre-Planting Trichoderma asperellum Application for Bocontrol of Macrophomina phaseolina in Screenhouse-Grown Cowpea(Tropical Journal of Natural Product Research, 2025) Oyesola, Olusola L.; Kinge, Tonjock R.; Kolade, Olufisayo A.; Obembe, Olawole O.Cowpea is recognised for its importance as a food and forage crop for animals. However, Macrophomina phaseolina, a fungus that causes pre-harvest crop loss, affects its production. In this study, Trichoderma was employed as an alternative to synthetic fungicides that negatively impact biodiversity to manage rot disease in cowpea. Three strains of Trichoderma asperellum were isolated from the soil. The spore suspensions of the Trichoderma strains were formulated into seven treatment combinations and applied to the cowpea soil before planting to investigate their biocontrol potential on M. phaseolina and their effects on cowpea biomass. The result showed that Trt3 (54.5417 cm), Trt1 (54.0625 cm) and Trt4 (52.8250 cm) had higher plant height than the negative control (M. phaseolina only (44.9667 cm)). Also, Trt7 (0.5446 cm) and Trt5 (0.5313 cm) had a higher stem girth performance than in the negative control (M. phaseolina only (0.3333 cm)), while Trt7 (24.958), Trt3 (21.417) and Trt6 (20.083) recorded a higher leaf number than in the negative control (M. phaseolina only (8.833)). Zero disease incidence was observed in Trt3 (0%) and Trt7 (0%) upon treatment with the Trichoderma formulations. Zero disease severity was recorded in Trt3 and Trt7 (0%), compared to the negative controls, which displayed 100% disease incidence and severity. The pre-planting Trichoderma application enhanced cowpea biomass and reduced disease incidence and severity compared to the negative control. Therefore, Trichoderma is an effective bioagent for controlling diseases caused by M. phaseolina in cowpea and stimulating its overall performance.Item Exciting Times for Cowpea Genetic Transformation Research(INSInet Publication) Obembe, Olawole O.Item Expression of an expansin carbohydrate-binding module affects xylem and phloem formation(Academic Journals) Obembe, Olawole O.Item Expression of the C-terminal family 22 carbohydratebinding module of xylanase 10B of Clostridium themocellum in tobacco plant(Academic Journals) Obembe, Olawole O.Item HIGH FREQUENCY MULTIPLE SHOOTS INDUCTION AND PLANT REGENERATION IN SIX ELITE INDIAN COTTON CULTIVARS(SENRA Academic Pblishers) Obembe, Olawole O.Item In silico analysis of compounds characterized from ethanolic extract of Cucurbita pepo with NF-κB-inhibitory potential(A Journal of the Bangladesh Pharmacological Society) Obembe, Olawole O.Item Irregular deposition of cell wall polymers resulting from defective cellulose synthase complexes(Indian Society for Education and Environment (iSee)) Obembe, Olawole O.Item Molecular analysis and phenotype characterization of the progeny of two antisense potato plants(2008 Academic Journals) Obembe, Olawole O.Item Morphological intraspecific variabilities in African yam bean (AYB) (Sphenostylis stenocarpa Ex. A. Rich) Harms(Academic Journals) Obembe, Olawole O.Item Offspring of the crosses of two anti-sense potato plants exhibit additive cellulose reduction(Academic Journals) Obembe, Olawole O.Item Post-germination Application of Trichoderma asperellum for the Biocontrol of Macrophomina phaseolina in Cowpea(Tropical Journal of Natural Product Research Vol 9, Issue 12,, 2025) Oyesola, Olusola L.; Kinge, Tonjock R.; Kolade, Olufisayo A.; Obembe, Olawole O.Cowpea (Vigna unguiculata (L.) Walp.) serves as a food source for humans and forage for animals. However, its production is affected by disease-causing fungi, of which Macrophomina phaseolina is a significant pathogen. Trichoderma was employed as a biofungicide to manage the disease in the screenhouse. Three strains of Trichoderma asperellum were isolated from the soil. The fungal spore suspensions of the Trichoderma strains were prepared, formulated into seven different treatment combinations, and applied to the cowpea potted soil five days after the germination of the cowpea to investigate their biocontrol ability on M. phaseolina and assess their effects on cowpea growth. The experiment's results showed that cowpea plants treated with T. asperellum differed significantly in plant height, stem girth, and leaf number compared to those treated with M. phaseolina alone (p < 0.05). Trt3 (54.6815 cm), Trt1 (54.0125 cm), and Trt5 (52.9375 cm) gave a higher plant height than in control 1 (M. phaseolina-treated cowpea - 44.9667 cm). Also, Trt7 (0.5413) and Trt3 (0.5258) gave a higher stem girth than in control 1 (M. phaseolina-treated cowpea - 0.3333 cm), while Trt6 (20.292) gave a higher leaf number than in control 1 (M. phaseolina-treated cowpea - 8.833). Additionally, Trt3 and Trt7 exhibited disease incidences of 22% and 67%, respectively, compared to control 1, which had a 100% incidence. Meanwhile, Trt7 showed 8% disease severity, compared to control 1, which had 100%. Therefore, post-germination Trichoderma application proved to be an effective strategy for controlling M. phaseolina, and it also has the potential to enhance cowpea biomass for sustainable food security