Characterization of Nanoformulations from Montmorillonite clay for the decontamination of zearalenonein cereals using X-ray Diffraction Technique
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Description
Montmorillonite clay due to its abundance and environmental friendliness has several
industrial applications among which are the adsorption of mycotoxins in foods and feed. The
mycotoxin; zearalenone (ZEA) is oestrogenic and has been implicated in hormonal and
reproductive issues for both man and animals. Thus, nanoformulations from Montmorillonite
clay and Cymbopogoncitratus (lemongrass) extracts were developed for the reduction of the toxin
in millet. The various formulations include: Montmorillonite clay to which extract of
Cymbopogoncitratus(lemon grass essential oil)has been incorporated (Mont-LGEO),
Montmorillonite clay mixed with C. citratus(lemon grass)powder (Mont-LGP) and
Montmorillonite clay washed with NaCl (Mont-Na). Pure Montmorillonite clay (Mont) and C.
citratus (lemon grass) powder (LGP) served as the controls for the treatment. Each of these
compositions were applied to millet samples at a concentration of 8 % and 12% and stored for 4
weeks. XRD patterns for compositions containing Montmorillonite clay revealed major peaks at
2-Theta value of 20.06° representing Montmorillonite and 26.56° and 68.53°representing quartz.
The level of ZEA in all treated samples was quantified after 4 weeks using the Liquid
Chromatography tandem mass spectrophotometer LC-MS/MS. All the compositions were
effective in the decontamination of zearalenone. Furthermore, C. citratus powder was the most
effective in the decontamination of zearalenone in the cereal after 4 weeks.
Keywords
QH301 Biology, QR Microbiology