Greener Technology of Producing Polyhydroxyalkanoate using Anthracene as Carbon Source
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Description
The study investigated the potential of Bacillus cereus AAR-1 (OQ999178) to simultaneously
degrade anthracene, a toxic environmental pollutant, and produce polyhydroxyalkanoate (PHA), an ecofriendly
and sustainable biopolymer. Using a Taguchi L16 (4*3) array for optimization, it was found that
a 10% seed inoculum, grown for 8 days in a minimal salt medium containing 400 ppm of anthracene and
2 g/L of NH4Cl as carbon and nitrogen sources, respectively, maximized anthracene degradation and
PHA accumulation by B. cereus AAR-1. The bacterial biomass had a colony count of 1 x 106 cfu/ml and
produced 286 mg/L of biopolymer, as extracted using a hypochlorite-chloroform solvent method. Fouriertransform
infrared spectroscopy (FTIR) analysis confirmed the biopolymer as PHA. This study identifies
a key hydrocarbon-degrading bacterium at a municipal dumpsite, which plays a significant role in
environmental biotechnology by supporting cleaner and greener technologies. This contribution aligns
with the goals of SDG 12 and 14
Keywords
TN Mining engineering. Metallurgy, TP Chemical technology