EFFECTIVE ALKALINE PEROXIDE OXIDATION PRETREATMENT OF SHEA TREE SAWDUST FOR THE PRODUCTION OF BIOFUELS: KINETICS OF DELIGNIFICATION AND ENZYMATIC CONVERSION TO SUGAR AND SUBSEQUENT PRODUCTION OF ETHANOL BY FERMENTATION USING Saccharomyces cerevisiae
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Description
Shea tree sawdust delignification kinetic data during alkaline peroxide pretreatment were
investigated at temperatures of 120 °C, 135 °C, and 150 °C. The activation energy during delignification was
76.4 kJ/mol and the Arrhenius constant was calculated as 8.4 x 106 per min. The reducing sugar yield for the
treated to the untreated biomass was about 22-fold. Enzymatic hydrolysis conditions studied were; time (72 h
and 96 h), substrate concentration (20, 30, 40, and 50 g/L), and enzyme loadings (10, 25, 40, 50 FPU/g dry
biomass), which showed the optimum conditions of 96 h, 40 g/L, and 25 FPU/g dry biomass at 45 °C hydrolysis
temperature. At the optimized enzymatic hydrolysis conditions, the reducing sugar yield was 416.32 mg
equivalent glucose/g treated dry biomass. After 96 h fermentation of treated biomass, the ethanol obtained at
2% effective cellulose loading was 12.73 g/L. Alkaline peroxide oxidation pretreatment and subsequent
enzymatic hydrolysis improved the ethanol yield of the biomass.
Keywords
QH301 Biology, TP Chemical technology