Expression of the C-terminal family 22 carbohydratebinding module of xylanase 10B of Clostridium themocellum in tobacco plant
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Carbohydrate-binding modules have been shown to alter plant cell wall structural architecture. Hence,
they have the potential application of being used to engineer the plant to produce tailor-made natural
fibers in the cell wall. The Clostridium thermocellum xylanase, Xyn10B, contains two CBMs that belong
to family 22 (CBM22). The C-terminal CBM22-2 of the glycoside hydrolase (GH) 10 had been
characterized to interact with xylan, a major hemicellulosic component in the secondary cell wall of
plants. In this work, the expression of the CBM22-2 in transgenic tobacco plants was evaluated.
Histological examinations of the transgenic stems did not reveal marked cell wall phenotype. In
addition, there were no observable changes in the height or the appearance of the transgenic plants
expressing the CBM22-2 module. The results indicate that the family 22 carbohydrate binding module is
not a potential candidate for use in in planta modification of the cell wall.
Keywords
QK Botany