Suitability of spectrophotometric assay for determination of honey microbial inhibition
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Description
Commonly used methods for determination of antibacterial potency of honey are usually
the disc, agar well diffusion and dilution plate assay which had shown various demerit of
inaccuracies and impreciseness. Therefore, the suitability of spectrophotometric assay for
determination of honey inhibitory activity is evaluated in this study. Honeys from
different sources in southwest states in Nigeria were assayed for antibacterial activity
using 96-well micro-titre plate spectrophotometric methods to determine the minimum
inhibitory concentration (MIC) against enteric bacteria strains. The honey inhibition assay
of Nigerian honeys tested against enteric bacilli showed more than 90% inhibitory
activity. Among all the honeys sample assayed, only two honeys reveal a very low MIC
of 31.25 and 125mg/mL. The use of spectrophotometry is a precise method to determine
honey inhibitory rate and it is proven to be suitable highly sensitive, reproducible,
specific, reduced cost, fewer amounts of sample and reagent are required
Keywords
QH301 Biology, QR Microbiology